团队简介：主要从事功能微生物筛选、开发及应用及功能天然产物的绿色生物制造等领域研究。近年来，承担国家自然科学基金面上（青年）项目、江苏省自然科学基金面上（青年）项目、江苏省高等学校自然科学研究面上项目、企业委托攻关技术等各类项目18项，在Biosensors & bioelectronics、Applied and Environmental Microbiology、Bioresource Technology等期刊上发表高水平学术论文26篇。
团队骨干：
研究内容：
1.氮限制条件下全局调控因子NtrC正向调控荚膜异多糖酸合成机制：通过转录组学与蛋白组学测序技术构建NtrC调控荚膜异多糖酸合成网络；再结合染色体免疫共沉淀测序、DNase I足迹实验及基因同框缺失等方法确定并验证影响荚膜异多糖酸合成的关键NtrC结合位点，揭示氮限制条件下NtrC调控荚膜异多糖酸合成机制，可为同类微生物多糖氮限制条件下合成调控机制的解析提供理论基础。
NtrC调控荚膜异多糖酸合成预测网络
2.Zn(Ⅱ)解除细菌β-葡聚糖胞外锚定的机理：以贝莱斯芽孢杆菌为研究对象，探究锌离子解除多糖胞外锚定的机理：结合转录组差异基因分析、蛋白功能分析，筛选锌指蛋白调控作用下能够解除多糖胞外锚定的解锚蛋白；通过基因敲除、回补、异源表达等手段验证解锚蛋白的功能；通过蛋白互作、分子对接、发酵液外源添加解锚蛋白等方式研究解锚定蛋白的作用机理。阐明Zn(Ⅱ)解除细菌β-葡聚糖胞外锚定的机制，为工业化生产β-葡聚糖或其他微生物多糖的分离提供理论基础和方法借鉴。
革兰氏阳性菌β-葡聚糖生物合成途径（Wzx/Wzy-依赖通路）
3.DNA甲基化影响CRISPRCas12a切割活性的分子机制：深入探究DNA甲基化如何调控CRISPR/Cas12a的活性与特异性，阐明甲基化对Cas12a识别和切割DNA能力的具体影响揭示甲基化调节Cas12a活性作用机制，明确甲基化如何影响基因组编辑的效率，以及表观遗传修饰与CRISPR技术之间的相互作用。
甲基化对 CRISPR/Cas12a 系统活性和特异性研究
4.木质素来源苯醌抑制酿酒酵母乙醇发酵的机理：在细胞层面对苯醌胁迫下酿酒酵母的细胞膜损伤进行分析，揭示细胞膜组分变化与其特性改变之间的关系；从代谢角度探究苯醌胁迫下胞内活性氧自由基积累的机制；结合组学分析，深入挖掘苯醌胁迫下细胞膜、活性氧自由基代谢等通路中的关键基因；构建苯醌抗逆重组菌株，并在富含苯醌的木质纤维素体系验证关键基因的功能，解析木质素来源苯醌对酿酒酵母的抑制机理，为其它菌株的苯醌抗逆强化改造提供技术支持和理论依据。
酿酒酵母的苯醌胁迫抗逆强化策略
5.2-甲基丁酸拮抗辣椒疫霉作用机制：通过分析微生物源挥发性物质（VOCs）2-甲基丁酸对菌株生长特性、细胞壁和细胞膜结构、菌株侵染性、致病毒力因子活性等方面的影响，考察菌株生理、致病性变化与2-甲基丁酸拮抗辣椒疫霉之间的关系；基于转录组和蛋白组学分析，结合生理、致病性结果，挖掘潜在的关键基因，系统解析2-甲基丁酸拮抗辣椒疫霉的作用机制；利用CRISPR/Cas9等基因编辑技术进行功能验证，明确关键基因在2-甲基丁酸拮抗辣椒疫霉中的作用机制，为2-甲基丁酸作为新型生物源杀菌剂及辣椒疫霉潜在药物靶标的挖掘提供理论依据。
微生物源VOCs对植物病原菌的拮抗作用
6.射频诱导低水分基质中沙门氏菌亚致死损伤及胁迫耐受性变化机制：通过分析射频加热和基质含水率对非膜损伤亚致死细胞的损伤特性和胁迫耐受性的影响；测定射频诱导的非膜损伤亚致死细胞对非热胁迫的耐受性，并从细胞水平阐明对ε-PL胁迫耐受性降低机理；通过组学技术分析正常细胞、ε-PL作用前后的非膜损伤亚致死细胞中差异表达的基因和蛋白，从分子水平揭示非膜损伤亚致死细胞ε-PL耐受性降低机制。为开发射频和非热方法联合技术实现低水分食品高效杀菌提供理论支撑。
射频诱导低水分基质沙门氏菌亚致死损伤及胁迫耐受性变化机制
代表性论文：
1.Bao Tang#, Lingtian Wu#, Jinzi Wang, Weibo Sun, Yancun Zhao, Fengquan Liu. Separation of heat-stable antifungal factor from Lysobacter enzymogenes fermentation broth via photodegradation and macroporous resin adsorption [J]. Frontiers in Microbiology,2021, 12: 663065.
2.Qiao Meng#, Chenghui Lu#, Hua Gao, Guoxuan Chen, Lingtian Wu*, Jinnan Wu, Sha Li, Bing-Fang He. Efficient biosynthesis of exopolysaccharide from Jerusalem artichoke using a novel strain of Bacillus velezensis LT-2 [J]. Bioresource Technology, 2021, 320: 124346.
3.Hua Gao#, Chenghui Lu#, Hui Wang, Liying Wang, Yang Yang, Tong Jiang, Sha Li, Delei Xu, Lingtian Wu*. Production exopolysaccharide from Kosakonia cowanii LT-1 through solid-state fermentation and its application as a plant growth promoter[J]. International Journal of Biological Macromolecules, 2020, 150: 955-964.
4.Xingyu Lu#, Qian Zhong#, Jian Liu, Fulin Yang, Chenghui Lu, Huan Xiong, Sha Li, Yibo Zhu, Lingtian Wu*. Efficient expression of chondroitinase ABC I for specific disaccharides detection of chondroitin sulfate [J]. International Journal of Biological Macromolecules, 2020, 143: 41-48.
5.Yuanmei Xu, Xiangyu Guan, Shaojin Wang*. Synergistic bactericidal mechanisms of RF energy simultaneously combined with cinnamon essential oil or epsilon-polylysine against Salmonella revealed at cellular and metabolic levels [J]. International Journal of Food Microbiology,2024, 408: 110447.
6.Yuanmei Xu, Juanjuan Xu, Gaoji Yang, Xiangyu Guan, Rui Li, Shaojin Wang*. Combined effects of intermittent radio frequency heating with cinnamon oil vapor on microbial control and quality changes of alfalfa seeds [J]. International Journal of Food Microbiology,2022, 367: 109586.
7.Yuanmei Xu, Rui Li, Kui Li, Jiangtao Yu, Junqing Bai, Shaojin Wang*. Inactivation of inoculated Salmonella and natural microflora on two kinds of edible seeds by radio frequency heating combined with cinnamon oil vapor [J]. LWT-Food Science and Technology,2022,154: 112603.
8.Feng Chen, Guodong Li, Chun Wu, Ling Wang, Chung-Nga Ko, Dik-Lung Ma*, Chung-Hang Leung*. Interference reduction biosensing strategy for highly sensitive microRNA detection [J]. Analytical Chemistry,2022, 94: 4513-4521.
9.Feng Chen, Guodong Li, Chun Wu, Wanhe Wang, Dik-Lung Ma*, and Chung-Hang Leung*. A rapid and label-free DNA-based interference reduction nucleic acid amplification strategy for viral RNA detection [J]. Biosensors & bioelectronics,2022, 198: 113829.
10.Feng Chen, Guodong Lia, Hao Liub, Chung-Hang Leunga*, and Dik-Lung Ma*. G-quadruplex-based detection of glyphosate in complex biological systems by a time-resolved luminescent assay [J]. Sensors and Actuators B: Chemical,2020, 320: 128393.
11.Zhao Yan, Jian Zhang, Jie Bao*. Increasing cellulosic ethanol production by enhancing phenolic tolerance of Zymomonas mobilis in adaptive evolution [J]. Bioresource Technology,2021, 329: 124926.
12.Zhao Yan#, Xiaochuang Gao#, Qiuqiang Gao, Jie Bao*. Mechanism of tolerance to the lignin-derived inhibitor p-benzoquinone and metabolic modification of biorefinery fermentation strains [J]. Applied and Environmental Microbiology,2019, 85: e01443-19.
13.Lishuang Nie, Tianyi Wei, Mingming Cao, Yunbin Lyu, Shaochen Wang, Zhiyang Feng*. Biosynthesis of coelulatin for the methylation of anthraquinone featuring HemN-like radical S-adenosyl-L-methionine enzyme [J] .Frontiers in Microbiology,2022,13:1040900.
14.Meilin Zhao, DingChang Shi , XinYao Lu , Hong Zong, BinZhuge*, Hao Ji. Ethanol fermentation from non-detoxified lignocellulose hydrolysate by a multi-stress tolerant yeast Candida glycerinogenes mutant[J]. Bioresource Technology, 2019, 273: 634-640.
15.Meilin Zhao, DingChang Shi , XinYao Lu , Hong Zong, BinZhuge*.Co-production of 1,2,4-butanetriol and ethanol from lignocellulose hydrolysate [J]. Bioresource Technology, 2019, 282: 433-438.
